Normal view MARC view ISBD view

DNA sequencing III : dealing with difficult templates / DNA sequencing three DNA sequencing 3 edited by Jan Kieleczawa. - Sudbury, Mass. : Jones and Bartlett Publishers, c2009. - xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cm.

Includes bibliographical references and index.

Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.

ISBN: 9780763742973 (alk. paper) 076374297X (alk. paper)

LCCN: 2007042261

Nat. Bib. Agency Control No.: 101318394 DNLM

Subjects--Topical Terms:
DNA--Analysis.
Nucleotide sequence--Methodology.
Sequence Analysis, DNA--methods.
Base Sequence.
Templates, Genetic.

LC Class. No.: QP624 / .D17492 2009

Dewey Class. No.: 572.86 KIE

National Library of Medicine Call No.: 2008 F-790 QU 450 / D62971 2009