DNA sequencing III :

Additional authors: Kieleczawa, Jan.
Published by : Jones and Bartlett Publishers, (Sudbury, Mass. :) Physical details: xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cm. ISBN:9780763742973 (alk. paper); 076374297X (alk. paper). Year: 2009
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Item type Current library Call number Status Date due Barcode
Books Books Cental Instituite of Fisheries Education
572.86 KIE (Browse shelf(Opens below)) Available 22224

Includes bibliographical references and index.

Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.

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