Transmembrane signaling protocols /

Includes bibliographical references and index.

Transmembrane signaling by G protein-coupled receptors / Louis M. Luttrell -- Crosstalk coregulation mechanisms of G protein-coupled receptors and receptor tyrosine kinases / Kanchana Nataranjan and Bradford C. Berk -- Protein-protein interactions in signaling cascades / Bruce J. Mayer -- Biological role of the CXCR4-SDF-1 axis in normal human hematopoietic cells / Marcin Majka and Mariusz Z. Ratajczak -- Functional expression of CXCR4 in Saccharomyces cerevisiae in the development of powerful tools for pharmacological characterization of CXCR4 / Zi-xuan Wang, James R. Broach, and Stephen C. Peiper -- Characterization of constitutively active mutants of G protein-coupled receptors / Jean-Marc Navenot, Zi-xuan Wang, and Stephen C. Peiper -- G protein-coupled receptor dimerization and signaling / Mario Mellado ... [et al.] -- Real-time analysis of G protein-coupled receptor signaling in live cells / Venkatakrishna R. Jala and Bodduluri Haribabu -- Isolation of membrane rafts and signaling complexes / Kathleen Boesze-Battaglia -- Methods for the study of signaling molecules in membrane lipid rafts and caveolae / Rennolds S. Ostrom and Paul A. Insel --

Bioluminescence resonance energy transfer to monitor protein-protein interactions / Tarik Issad and Ralf Jockers -- Identification of interacting proteins using the yeast two-hybrid screen / Kelly L. Jordan-Sciutto and Marshall B. Montgomery -- Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays / Hyun Woo Lee, Jaewon Ko, and Eunjoon Kim -- Clustering assay for studying the interaction of membrane proteins with PDZ domain proteins / Jaewon Ko and Eunjoon Kim -- Mammalian cell microinjection assay to study the function of rho family guanosine triphosphatases / Ritu Garg and Anne J. Ridley -- Affinity-based assay of Rho guanosine triphosphatase activation / Mary Stofega, Celine DerMardirossian, and Gary M. Bokoch -- Assay of phospholipase D activity in cell-free systems / Shankar S. Iyer and David J. Kusner -- Reconstitution system based on cytosol-depleted cells to study the regulation of phospholipase D / Amanda Fensome-Green and Shamshad Cockcroft -- Analysis of global gene expression profiles activated by chemoattractant receptors / Fernando O. Martinez and Massimo Locati -- Genetic reconstitution of bone marrow for the study of signal transduction ex vivo / Martha S. Jordan -- Proteomic analysis of human neutrophils / George Lominadze ... [et al.].