TY - BOOK AU - Espina,Virginia TI - Molecular profiling: methods and protocols T2 - Methods in molecular biology, SN - 9781493969890 AV - RB43.7 .M652 2017 U1 - 616.0756 ESP 23 KW - Molecular diagnosis KW - Laboratory manuals KW - Pathology, Molecular KW - Molecular Diagnostic Techniques KW - methods KW - Neoplasms KW - therapy KW - Tissue Array Analysis KW - fast KW - Laboratory Manuals N1 - Includes bibliographical references and index; Tumor staging and grading : a primer; Stacy M. Telloni --; Innovations in clinical trial design in the era of molecular profiling; Julia D. Wulfkuhle, Alexander Spira, Kirsten H. Edmiston, and Emanuel F. Petricoin III --; Personalized medicine : ethical aspects; G. Terry Sharrer --; Antibody validation by Western Blotting; Michele Signore, Valeria Manganelli, and Alex Hodge --; Scanning electron microscopy sample preparation and imaging; Jenny Ngoc Tran Nguyen and Amanda M. Harbison --; One-step preservation and decalcification of bony tissue for molecular profiling; Claudius Mueller, Michael G. Harpole, and Virginia Espina --; Application of hydrogel nanoparticles for the capture, concentration, and preservation of low-abundance biomarkers; Ruben Magni and Alessandra Luchini --; Using laser capture microdissection to isolate cortical laminae in nonhuman primate brain; Brian A. Corgiat and Claudius Mueller --; Western blot techniques; Brianna Kim --; ELISA for monitoring nerve growth factor; Justin B. Davis --; Reverse phase protein microarrays; Elisa Baldelli, Valerie Calvert, Alex Hodge, Amy VanMeter, Emanuel F. Petricoin III, and Mariaelena Pierobon --; Clustering and network analysis of reverse phase protein array data; Adam Byron --; PCR : identification of genetic polymorphisms; Amanda M. Harbison and Jenny Ngoc Tran Nguyen --; Microsatellite analysis for identification of individuals using bone from the extinct steller's sea cow (Hydrodamalis gigas); Jeffery F. Warner, Michael G. Harpole, and Lorelei D. Crerar --; Somatic DNA mutation analysis; Anthony O'Grady and Robert Cummins --; Optimization of immunostaining for prospective image analysis; Scott M. Lawrence and Yelena G. Golubeva --; Fluorescence in situ hybridization of cells, chromosomes, and formalin-fixed paraffin-embedded tissues; Ahmad Alamri, Jun Yeb Nam, and Jan K. Blancato --; High-resolution image stitching as a tool to assess tissue-level protein distribution and localization; Bryan A. Millis and Matthew J. Tyska --; Mass spectrometry-based biomarker discovery; Weidong Zhou, Emanuel F. Petricoin III, and Caterina Longo --; Quantitative mass spectrometry by isotope dilution and multiple reaction monitoring (MRM); Paul Russo, Brian L. Hood, Nicholas W. Bateman, and Thomas P. Conrads --; LC-mass spectrometry for metabolomics; Allyson L. Dailey --; Metabolomic Bioinformatic Analysis; Allyson L. Dailey --; Stable isotope quantitative N-glycan analysis by liquid separation techniques and mass spectrometry; Stefan Mittermayr, Simone Albrecht, Csaba Váradi, Silvia Millán-Martín, and Jonathan Bones --; Grant writing tips for translational research; Lindsay Wescott, Michael Laskofski, Donna Senator, and Carly Curran --; Inventions and patents : a practical tutorial; Hina Mehta, Lille Tidwell, and Lance A. Liotta --; Product development and commercialization of diagnostic or life science products for scientists and researchers; Meghan M. Alonso UR - http://www.springer.com/us/book/9781493969890 UR - http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-4939-6990-6 ER -