TY  - BOOK
AU  - Kieleczawa,Jan
TI  - DNA sequencing III: dealing with difficult templates 
SN  - 9780763742973 (alk. paper)
AV  - QP624 .D17492 2009
U1  - 572.86 KIE 22
PY  - 2009///
CY  - Sudbury, Mass.
PB  - Jones and Bartlett Publishers
KW  - DNA
KW  - Analysis
KW  - Nucleotide sequence
KW  - Methodology
KW  - Sequence Analysis, DNA
KW  - methods
KW  - Base Sequence
KW  - Templates, Genetic
N1  - Includes bibliographical references and index; Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates
UR  - http://www.loc.gov/catdir/toc/ecip082/2007042261.html
ER  -