TY  - BOOK
AU  - Clokie,Martha R.J.
AU  - Kropinski,Andrew M.
TI  - Bacteriophages :Vol-2: methods and protocols 
T2  - Methods in molecular biology,
SN  - 9781588296825 (v.1) (hbk.)
AV  - QR342 .B34 2009
U1  - 576.6482 CLO 22
PY  - 2009///
CY  - New York
PB  - Humana Press
KW  - Bacteriophages
KW  - Research
KW  - Methodology
KW  - physiology
KW  - Laboratory Manuals
KW  - isolation & purification
KW  - Genomics
KW  - methods
KW  - Bactériophages
KW  - ram
N1  - Includes bibliographical references and index; v.1. Isolation, characterization, and interactions -- v.2. Molecular and applied aspects; v. 1. Isolation, characterization, and interactions: v. 1. Sec. 1. Methods for the isolation of viruses from environmental samples / K. Eric Wommack ... [et al.] -- 2. Bacteriophage enrichment from water and soil / Rohan Van Twest and Andrew M. Kropinski -- 3. Isolation of phage via induction of lysogens / Raúl R. Raya and Elvira M. Hébert -- 4. Isolation of cyanophages from aquatic environments / Andrew D. Millard -- 5. Isolation of viruses from high temperature environments / Jennifer Fulton, Trevor Douglas and Mark Young -- 6. Isolation of novel large and aggregating bacteriophages / Philip Serwer ... [et al.] -- v. 1. Sec. 2. Bacteriophage characterization: 7. Enumeration of bacteriophages by double agar overlay plaque assay / Andrew M. Kropinski ... [et al.] -- 8. Enumeration of bacteriophages by the direct plating plaque assay / Amanda Mazzocco ... [et al.] -- 9. Enumeration of bacteriophages using the small drop plaque assay system / Amanda Mazzocco ... [et al.] -- 10. Determination of virus abundance by epifluorescence microscopy / Alice C. Ortmann and Curtis A. Suttle -- 11. Enumeration of bacteriophages using flow cytometry / Corina P. D. Brussaard -- 12. Basic phage electron microscopy / Hans-W. Ackermann -- 13. Phage classification and characterization / Hans-W. Ackermann -- 14. Phage host range and efficiency of plating / Elizabeth Kutter -- 15. Measurement of the rate of attachment of bacteriophage to cells / Andrew M. Kropinski -- 16. Measurement of the bacteriophage inactivation kinetics with purified receptors / Andrew M. Kropinski -- 17. Bacteriophage plaques / Stephen T. Abedon and John Yin -- 18. Practical methods for determining phage growth parameters / Paul Hyman and Stephen T. Abedon -- 19. Phage production and maintenance of stocks, including expected stock lifetimes / Louis-Charles Fortier and Sylvain Moineau; v. 1. Sec. 3. Bacteriophage-host interactions: 20. Construction of phage mutants (general)/  Robert Villafane--  21. Modifying bacteriophage l with recombineering / Lynn C. Thomason, Amos B. Oppenheim, and Donald L. Court -- 22. Identification and isolation of lysogens with induced prophage / Jonathan Livny, Christopher N. LaRock and David I. Friedman -- 23. Generalized transduction / Anne Thierauf, Gerardo Perez, and Stanley Maloy -- 24. Preparation and characterization of anti-phage serum / Thomas E. Waddell ... [et al.] -- 25. Generalized transduction by lytic bacteriophages / Thomas E. Waddell ... [et al.]; v. 2. Molecular and applied aspects: v. 2. Sec. 1. Bacteriophage genomics: 1. Preparation of bacteriophage lysates and pure DNA / Derek John Juan Pickard -- 2. Approaches to the compositional analysis of DNA / Richard A. Manderville and Andrew M. Kropinski -- 3. Determination of bacteriophage genome size by pulsed-field gel electrophoresis / Erika Lingohr, Shelley Frost, and Roger P. Johnson -- 4. Preparation of a phage DNA fragment library for whole genome shotgun sequencing / Elizabeth J. Summer -- 5. PCR and partial sequencing of bacteriophage genomes / Martha Clokie -- 6. In Silico identification of genes in bacteriophage DNA / Andrew M. Kropinski ... [et al.] -- 7. Determining DNA packaging strategy by analysis of the termini of the chromosomes in tailed-bacteriophage virions / Sherwood R. Casjens and Eddie B. Gilcrease -- 8. In Silico characterization of DNA motifs with particular reference to promoters and terminators / Rob Lavigne, Andre Villegas and Andrew M. Kropinksi -- 9. Molecular phylogenetics / David A. Walsh and Adrian K. Sharma -- v.2 Sec. 2. Bacteriophage transcriptomics and proteomics 10. Preparation of RNA from bacteria infected with bacteriophages, a case study from the marine unicellular synechococcus sp. WH7803 Infected by Phage S-PM2 / Jinyu Shan and Martha Clokie -- 11. Quantification of host and Phage mRNA expression during Infection using real-time PCR / Martha R. J. Clokie -- 12. Oligonucleotide microarrays for bacteriophage expression studies / Andrew D. Millard and Bella Tiwari -- 13. Purification of bacteriophages and SDS-PAGE analysis of phage structural proteins from ghost particles / Pascale Boulanger -- 14. Phage proteomics, applications of mass spectrometry / Rob Lavigne, Pieter-Jan Ceyssens and J. Robben --; v. 2. Sec. 3: Community bacteriophage approaches: 15. Isolation independent methods of characterizing phage communities 1, strain typing using fingerprinting methods / Clemens Pausz, Jessica L. Clasen, and Curtis A. Suttle -- 16. Isolation independent methods of characterizing phage communities 2, characterizing a metagenome / K. Eric Wommack ... [et al.] -- v. 2. Sec. 4. Applied aspects of bacteriophage biology: 17. Phage typing / Irina Chirakadze, Ann Perets, and Rafiq Ahmed -- 18. A Genetic screen to identify bacteriophage lysins / Raymond Schuch, Vincent A. Fischetti and Daniel C. Nelson -- 19. General M13 phage display, M13 phage display in identification and characterization of protein-protein interactions / Kirsten Hertveldt, Tim Beliën, and Guido Volckaert -- 20. Isolation of monoclonal antibody fragments from phage display libraries / Mehdi Arbabi-Ghahroudi, Jamshid Tanha, and Roger MacKenzie -- 21. Internet resources of Interest to bacteriophage workers / Andrew M. Kropinski
ER  -