Basic cell culture protocols.

Includes bibliographical references and index.

Culture of primary adherent cells and a continuously growing non-adherent cell line / Cheryl D. Helgason -- Detection of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Eradication of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Authentication of scientific human cell lines / Wilhelm G. Dirks and Hans G. Drexler -- Cytogenetic analysis of cell lines / Roderick A.F. MacLeod and Hans G. Drexler -- Human and mouse hematopoeitic colony forming cell assays / Cindy L. Miller and Becky Lai -- Isolation and culture of murine macrophages / John Q. Davies and Siamon Gordon -- Isolation and culture of human macrophages / John Q. Davies and Siamon Gordon -- The development of T lymphocytes in mouse fetal thymus organ culture / Tomoo Ueno ... [et al.] -- In vitro generation of lymphocytes from embryonic stem cells / Renée F. de Pooter, Sarah K. Cho and Juan Carlos Zúniga-Pflücker -- Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman -- Generation of murine stromal cell lines / Robert A.J. Oostendorp, Kirsty Harvey and Elaine E. Dzierzak -- Culture of human and mouse mesenchymal cells / Emer Clarke -- Isolation, purification, and cultivation of rat and human keratinocytes / Frizell L. Vaughan and Ludmila I. Bernstam -- Isolation and culture of primary human hepatocytes / Edward L. LeCluyse ... [et al.] -- Primary kidney proximal tubule cells / Mary Taub -- Enzymatic dissociation and culture of normal human mammary tissue to detect progenitor activity / John Stingl, Joanne T. Emerman, and Connie J. Eaves -- Generation and differentiation of neurospheres from murine embryonic day 14 central nervous system tissue / Sharon A. Louis and Brent A. Reynolds -- Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells / Gabi Shefer and Zipora Yablonka-Reuveni -- Adult ventricular cardiomyocytes / Klaus-Dieter Schlüter and Daniela Schreiber -- Isolation and culture of primary endothelial cells / Bruno Larrivée and Aly Karsan -- Studying leukocyte rolling and adhesion in vitro under flow conditions / Susan L. Cuvelier and Kamala D. Patel -- Isolation and characterization of side population cells / Margaret A. Goodell, Shannon McKinney-Freeman and Fernando D. Camargo -- Scalable production of embryonic stem cell-derived cells / Stephen M. Dang and Peter W. Zandstra.

Minimum system requirements: Adobe reader 6.0 or later.