Parasite genomics protocols /

Includes bibliographical references and index.

Sequencing strategies for parasite genomes / Daniella Bartholomeu and Najib M. El-Sayed -- Annotation of parasite genomes / Matthew Berriman and Midori Harris -- Parasite genome databases and web-based resources / Christiane Hertz-Fowler and Neil Hall -- Expressed sequence tags: medium throughput protocols / Claire Whitton, Jennifer Daub, Marian Thompson, and Mark Blaxter -- Expressed sequence tags: analysis and annotation / John Parkinson and Mark Blaxter -- Positive selection scanning of parasite DNA sequences-- Winston A. Hide and Raphael D. Isokpehi -- Race and rage cloning in parasitic microbial eukaryotes / Bryony A. P. Williams and Robert P. Hirt -- Amplified (restriction) fragment length polymorphism (AFLP) analysis / Daniel K. Masiga and C. Michael R. Turner --

Minisatellites and MVR-PCR for the individual identification of parasite isolates / Annette MacLeod -- Analysis of differentially expressed parasite genes and proteins using transcriptomics and proteomics / Daniel C. Gare -- Gene expression studies using self-fabricated parasite cDNA microarrays / Karl F. Hoffmann and Jennifer M. Fitzpatrick -- DNA content analysis on microarrays / Upinder Singh, Preetam H. Shah, and Ryan C. MacFarlane -- Typing single nucleotide polymorphisms in toxoplasma gondii by allele-specific primer extension and microarray detection / Chunlei Su, Christian Hott, Bernard H. Brownstein, and L. David Sibley -- Transfection of the human malaria parasite plasmodium falciparum / Brendan S. Crabb, Melanie Rug, Tim-Wolf Gilberger, Jennifer K. Thompson, Tony Triglia, Alexander G. Maier, and Alan F. Cowman --

A PCR-based method for gene deletion and protein tagging in trypanosoma brucei / George K. Arhin, Shuiyuan Shen, Elisabetta Ullu, and Christian Tschudi -- Analysis of gene function in trypanosoma brucei using RNA interference / Appolinaire Djikeng, Shuiyuan Shen, Christian Tschudi, and Elisabetta Ullu --

In vitro shuttle mutagenesis using engineered mariner transposons / Kelly A. Robinson, Sophie Goyard, and Stephen M. Beverley -- Random mutagenesis strategies for construction of large and diverse clone libraries of mutated DNA fragments / Sudsanguan Chusacultanachai and Yongyuth Yuthavong -- Separation, digestion and cloning of intact parasite chromosomes embedded in agarose / Vanessa Leech, Michael A. Quail, and Sara E. Melville --

Chromosome fragmentation in leishmania / Pascal Dubessay, Christine Blaineau, Patrick Bastien, and Michel PagÅs -- FISH mapping for helminth genomes / Hirohisa Hirai and Yuriko Hirai -- Fiber-FISH: fluorescence in situ hybridization on stretched DNA / Klaus Ersfeld -- Yeast two-hybrid assay for studying protein-protein interactions / Ahmed Osman -- From genomes to vaccines for leishmaniasis / Carmel B. Stober.