Recombinant gene expression

Rev. ed. of: Recombinant gene expression protocols / edited by Rocky S. Tuan. c1997.

Includes bibliographical references and index.

Host cell compatibility in protein expression / James J. Greene -- Production of recombinant proteins / Laura A. Palomares, Sandino Estrada-Mondaca, and Octavio T. Ramírez -- Folding promoting agents in recombinant protein production / Beatrix Fahnert -- Back to basics / Paulina Balbás and Francisco Bolivar -- Alpha-complementation enabled t7 expression vectors and their use for the expression of recombinant polypeptides for protein transduction experiments / Mikhail F. Alexeyev, Viktoriya V. Pastukh, Inna N. Shokolenko, and Glenn L. Wilson -- Expression of recombinant alkaline phosphatase conjugates in Escherichia coli / Jean-Claude Boulain and Frédéric Ducancel -- Overexpression of chromosomal genes in Escherichia coli / Fernando Valle and Noemí Flores -- Chromosomal expression of foreign and native genes from regulatable promoters in Escherichia coli / Lu Zhou, Ke Zhang and Barry L. Wanner --

Plasmid vectors for marker-free chromosomal insertion of genetic material in Escherichia coli / Sylvie Le Borgne, Francisco Bolivar, and Guillermo Gosset -- Copy-control pBAC/oriV vectors for genomic cloning / Jadwiga Wild and Waclaw Szybalski -- Copy-control tightly regulated expression vectors based on pBAC/oriV / Jadwiga Wild and Waclaw Szybalski -- Cell-free protein synthesis with prokaryotic combined transcription-translation / James R. Swartz, Michael C. Jewett, and Kim A. Woodrow-Mumford -- Genetic tools for the manipulation of moderately halophilic bacteria of the family halomonadaceae / Carmen Vargas and Joaquín J. Nieto -- Gene transfer and expression of recombinant proteins in moderately halophilic bacteria / Amalia S. Afendra, Carmen Vargas, Joaquín J. Nieto and Constantin Drainas -- Recombinant protein production in antarctic gram-negative bacteria / Angela Duilio, Maria Luisa Tutino, and Gennaro Marino -- Recombinant protein production in yeasts / Danilo Porro and Diethard Mattanovich -- Controlled expression of homologous genes by genomic promoter replacement in the yeast Saccharomyces cerevisiae / Kevin J. Vestrepen and Johan M. Thevelein -- High-throughput expression in microplate format in saccharomyces cerevisiae / Caterina Holz and Christine Lang -- High-throughput expression in microplate format in Pichia pastoris / Mewes Bötner and Christine Lang -- Multiple gene expression by chromosomal integration and CRE-lox mediated marker recycling in saccharomyces cerevisiae / Björn Johansson and Bärbel Hahn-Hägerdal --

Three decades of fungal transformation / Vianey Olmedo-Monfil, Carlos Cortés-Penagos, and Alfredo Herrera-Estrella -- Three decades of fungal transformation / Sergio Casas-Flores, Teresa Rosales-Saavedra, and Alfredo Herrera-Estrella -- Gene transfer and expression in plants / Argelia Lorence and Robert Verpoorte -- Production of recombinant proteins by hairy roots cultured in plastic sleeve bioreactors / Fabricio Medina-Bolívar and Carole Cramer -- Engineering the chloroplast genome for hyperexpression of human therapeutic proteins and vaccine antigens / Shashi Kumar and Henry Daniell -- New selection marker for plant transformation / Barbara Leyman, Nelson Avonce, Matthew Ramon, Patrick Van Dijck, Johan M. Thevelein, and Gabriel Iturriaga -- Enhancer detection and gene trapping a functional genomics in plants / Gerardo Acosta-García, Daphné Autran, and Jean-Philippe Vielle-Calzada -- Gene transfer and expression in mammalian cell lines and transgenic animals / Felix Recillas-Targa -- Sustained heterologous transgene expression in mammalian and avian cell lines / Héctor Rincón-Arano and Felix Recillas-Targa -- Inducible gene expression in mammalian cells and mice / Wilfried Webber and Martin Fussenegger -- Flp-mediated integration of expression cassettes into FRT-tagged chromosomal lociin mammalian cells / Dagmar Wirth and Hansjörg Hauser -- Generation of high recombinant protein producing Chinese hamster ovary cells / Jean-Louis Goergen and Lucía Monaco -- Preparation of recombinant proteins in milk / Louis-Marie Houdebine.

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