Bacteriophages :Vol-2
Bacteriophages :Vol-2 methods and protocols /
edited by Martha R.J. Clokie, Andrew M. Kropinski.
- New York : Humana Press, 2009.
- 373p. ill. ; 26 cm.
- Methods in molecular biology, v. 501-502 1064-3745 ; Springer protocols .
- Methods in molecular biology (Clifton, N.J.) ; v. 501-502. .
Includes bibliographical references and index.
v.1. Isolation, characterization, and interactions -- v.2. Molecular and applied aspects. v. 1. Isolation, characterization, and interactions: v. 1. Sec. 1. Methods for the isolation of viruses from environmental samples / K. Eric Wommack ... [et al.] -- 2. Bacteriophage enrichment from water and soil / Rohan Van Twest and Andrew M. Kropinski -- 3. Isolation of phage via induction of lysogens / Raúl R. Raya and Elvira M. Hébert -- 4. Isolation of cyanophages from aquatic environments / Andrew D. Millard -- 5. Isolation of viruses from high temperature environments / Jennifer Fulton, Trevor Douglas and Mark Young -- 6. Isolation of novel large and aggregating bacteriophages / Philip Serwer ... [et al.] -- v. 1. Sec. 2. Bacteriophage characterization: 7. Enumeration of bacteriophages by double agar overlay plaque assay / Andrew M. Kropinski ... [et al.] -- 8. Enumeration of bacteriophages by the direct plating plaque assay / Amanda Mazzocco ... [et al.] -- 9. Enumeration of bacteriophages using the small drop plaque assay system / Amanda Mazzocco ... [et al.] -- 10. Determination of virus abundance by epifluorescence microscopy / Alice C. Ortmann and Curtis A. Suttle -- 11. Enumeration of bacteriophages using flow cytometry / Corina P. D. Brussaard -- 12. Basic phage electron microscopy / Hans-W. Ackermann -- 13. Phage classification and characterization / Hans-W. Ackermann -- 14. Phage host range and efficiency of plating / Elizabeth Kutter -- 15. Measurement of the rate of attachment of bacteriophage to cells / Andrew M. Kropinski -- 16. Measurement of the bacteriophage inactivation kinetics with purified receptors / Andrew M. Kropinski -- 17. Bacteriophage plaques / Stephen T. Abedon and John Yin -- 18. Practical methods for determining phage growth parameters / Paul Hyman and Stephen T. Abedon -- 19. Phage production and maintenance of stocks, including expected stock lifetimes / Louis-Charles Fortier and Sylvain Moineau. v. 1. Sec. 3. Bacteriophage-host interactions: 20. Construction of phage mutants (general)/ Robert Villafane-- 21. Modifying bacteriophage l with recombineering / Lynn C. Thomason, Amos B. Oppenheim, and Donald L. Court -- 22. Identification and isolation of lysogens with induced prophage / Jonathan Livny, Christopher N. LaRock and David I. Friedman -- 23. Generalized transduction / Anne Thierauf, Gerardo Perez, and Stanley Maloy -- 24. Preparation and characterization of anti-phage serum / Thomas E. Waddell ... [et al.] -- 25. Generalized transduction by lytic bacteriophages / Thomas E. Waddell ... [et al.]. v. 2. Molecular and applied aspects: v. 2. Sec. 1. Bacteriophage genomics: 1. Preparation of bacteriophage lysates and pure DNA / Derek John Juan Pickard -- 2. Approaches to the compositional analysis of DNA / Richard A. Manderville and Andrew M. Kropinski -- 3. Determination of bacteriophage genome size by pulsed-field gel electrophoresis / Erika Lingohr, Shelley Frost, and Roger P. Johnson -- 4. Preparation of a phage DNA fragment library for whole genome shotgun sequencing / Elizabeth J. Summer -- 5. PCR and partial sequencing of bacteriophage genomes / Martha Clokie -- 6. In Silico identification of genes in bacteriophage DNA / Andrew M. Kropinski ... [et al.] -- 7. Determining DNA packaging strategy by analysis of the termini of the chromosomes in tailed-bacteriophage virions / Sherwood R. Casjens and Eddie B. Gilcrease -- 8. In Silico characterization of DNA motifs with particular reference to promoters and terminators / Rob Lavigne, Andre Villegas and Andrew M. Kropinksi -- 9. Molecular phylogenetics / David A. Walsh and Adrian K. Sharma -- v.2 Sec. 2. Bacteriophage transcriptomics and proteomics 10. Preparation of RNA from bacteria infected with bacteriophages, a case study from the marine unicellular synechococcus sp. WH7803 Infected by Phage S-PM2 / Jinyu Shan and Martha Clokie -- 11. Quantification of host and Phage mRNA expression during Infection using real-time PCR / Martha R. J. Clokie -- 12. Oligonucleotide microarrays for bacteriophage expression studies / Andrew D. Millard and Bella Tiwari -- 13. Purification of bacteriophages and SDS-PAGE analysis of phage structural proteins from ghost particles / Pascale Boulanger -- 14. Phage proteomics, applications of mass spectrometry / Rob Lavigne, Pieter-Jan Ceyssens and J. Robben -- v. 2. Sec. 3: Community bacteriophage approaches: 15. Isolation independent methods of characterizing phage communities 1, strain typing using fingerprinting methods / Clemens Pausz, Jessica L. Clasen, and Curtis A. Suttle -- 16. Isolation independent methods of characterizing phage communities 2, characterizing a metagenome / K. Eric Wommack ... [et al.] -- v. 2. Sec. 4. Applied aspects of bacteriophage biology: 17. Phage typing / Irina Chirakadze, Ann Perets, and Rafiq Ahmed -- 18. A Genetic screen to identify bacteriophage lysins / Raymond Schuch, Vincent A. Fischetti and Daniel C. Nelson -- 19. General M13 phage display, M13 phage display in identification and characterization of protein-protein interactions / Kirsten Hertveldt, Tim Beliën, and Guido Volckaert -- 20. Isolation of monoclonal antibody fragments from phage display libraries / Mehdi Arbabi-Ghahroudi, Jamshid Tanha, and Roger MacKenzie -- 21. Internet resources of Interest to bacteriophage workers / Andrew M. Kropinski.
9781588296825 (v.1) (hbk.) 1588296822 (v.1) (hbk.) 9781603275644 (v. 2) (hbk.) 1603275649 (v. 2) (hbk.) 9781603271646 (v. 1) (eISBN) 1603271643 (v. 1) (eISBN) 9781603275651 (v. 2) (eISBN) 1603275657 (v. 2) (eISBN)
2008939449
GBA894232 bnb
101493772 DNLM 20090091124
Bacteriophages--Research--Methodology.
Bacteriophages.
Bacteriophages--physiology--Laboratory Manuals.
Bacteriophages--isolation & purification--Laboratory Manuals.
Genomics--methods--Laboratory Manuals.
Bactériophages.
QR342 / .B34 2009
576.6482 CLO
QR342 / .B339 2009
W1 ME9616J v. 501-502 2009 QW 25.5.B2 / B131 2009
Includes bibliographical references and index.
v.1. Isolation, characterization, and interactions -- v.2. Molecular and applied aspects. v. 1. Isolation, characterization, and interactions: v. 1. Sec. 1. Methods for the isolation of viruses from environmental samples / K. Eric Wommack ... [et al.] -- 2. Bacteriophage enrichment from water and soil / Rohan Van Twest and Andrew M. Kropinski -- 3. Isolation of phage via induction of lysogens / Raúl R. Raya and Elvira M. Hébert -- 4. Isolation of cyanophages from aquatic environments / Andrew D. Millard -- 5. Isolation of viruses from high temperature environments / Jennifer Fulton, Trevor Douglas and Mark Young -- 6. Isolation of novel large and aggregating bacteriophages / Philip Serwer ... [et al.] -- v. 1. Sec. 2. Bacteriophage characterization: 7. Enumeration of bacteriophages by double agar overlay plaque assay / Andrew M. Kropinski ... [et al.] -- 8. Enumeration of bacteriophages by the direct plating plaque assay / Amanda Mazzocco ... [et al.] -- 9. Enumeration of bacteriophages using the small drop plaque assay system / Amanda Mazzocco ... [et al.] -- 10. Determination of virus abundance by epifluorescence microscopy / Alice C. Ortmann and Curtis A. Suttle -- 11. Enumeration of bacteriophages using flow cytometry / Corina P. D. Brussaard -- 12. Basic phage electron microscopy / Hans-W. Ackermann -- 13. Phage classification and characterization / Hans-W. Ackermann -- 14. Phage host range and efficiency of plating / Elizabeth Kutter -- 15. Measurement of the rate of attachment of bacteriophage to cells / Andrew M. Kropinski -- 16. Measurement of the bacteriophage inactivation kinetics with purified receptors / Andrew M. Kropinski -- 17. Bacteriophage plaques / Stephen T. Abedon and John Yin -- 18. Practical methods for determining phage growth parameters / Paul Hyman and Stephen T. Abedon -- 19. Phage production and maintenance of stocks, including expected stock lifetimes / Louis-Charles Fortier and Sylvain Moineau. v. 1. Sec. 3. Bacteriophage-host interactions: 20. Construction of phage mutants (general)/ Robert Villafane-- 21. Modifying bacteriophage l with recombineering / Lynn C. Thomason, Amos B. Oppenheim, and Donald L. Court -- 22. Identification and isolation of lysogens with induced prophage / Jonathan Livny, Christopher N. LaRock and David I. Friedman -- 23. Generalized transduction / Anne Thierauf, Gerardo Perez, and Stanley Maloy -- 24. Preparation and characterization of anti-phage serum / Thomas E. Waddell ... [et al.] -- 25. Generalized transduction by lytic bacteriophages / Thomas E. Waddell ... [et al.]. v. 2. Molecular and applied aspects: v. 2. Sec. 1. Bacteriophage genomics: 1. Preparation of bacteriophage lysates and pure DNA / Derek John Juan Pickard -- 2. Approaches to the compositional analysis of DNA / Richard A. Manderville and Andrew M. Kropinski -- 3. Determination of bacteriophage genome size by pulsed-field gel electrophoresis / Erika Lingohr, Shelley Frost, and Roger P. Johnson -- 4. Preparation of a phage DNA fragment library for whole genome shotgun sequencing / Elizabeth J. Summer -- 5. PCR and partial sequencing of bacteriophage genomes / Martha Clokie -- 6. In Silico identification of genes in bacteriophage DNA / Andrew M. Kropinski ... [et al.] -- 7. Determining DNA packaging strategy by analysis of the termini of the chromosomes in tailed-bacteriophage virions / Sherwood R. Casjens and Eddie B. Gilcrease -- 8. In Silico characterization of DNA motifs with particular reference to promoters and terminators / Rob Lavigne, Andre Villegas and Andrew M. Kropinksi -- 9. Molecular phylogenetics / David A. Walsh and Adrian K. Sharma -- v.2 Sec. 2. Bacteriophage transcriptomics and proteomics 10. Preparation of RNA from bacteria infected with bacteriophages, a case study from the marine unicellular synechococcus sp. WH7803 Infected by Phage S-PM2 / Jinyu Shan and Martha Clokie -- 11. Quantification of host and Phage mRNA expression during Infection using real-time PCR / Martha R. J. Clokie -- 12. Oligonucleotide microarrays for bacteriophage expression studies / Andrew D. Millard and Bella Tiwari -- 13. Purification of bacteriophages and SDS-PAGE analysis of phage structural proteins from ghost particles / Pascale Boulanger -- 14. Phage proteomics, applications of mass spectrometry / Rob Lavigne, Pieter-Jan Ceyssens and J. Robben -- v. 2. Sec. 3: Community bacteriophage approaches: 15. Isolation independent methods of characterizing phage communities 1, strain typing using fingerprinting methods / Clemens Pausz, Jessica L. Clasen, and Curtis A. Suttle -- 16. Isolation independent methods of characterizing phage communities 2, characterizing a metagenome / K. Eric Wommack ... [et al.] -- v. 2. Sec. 4. Applied aspects of bacteriophage biology: 17. Phage typing / Irina Chirakadze, Ann Perets, and Rafiq Ahmed -- 18. A Genetic screen to identify bacteriophage lysins / Raymond Schuch, Vincent A. Fischetti and Daniel C. Nelson -- 19. General M13 phage display, M13 phage display in identification and characterization of protein-protein interactions / Kirsten Hertveldt, Tim Beliën, and Guido Volckaert -- 20. Isolation of monoclonal antibody fragments from phage display libraries / Mehdi Arbabi-Ghahroudi, Jamshid Tanha, and Roger MacKenzie -- 21. Internet resources of Interest to bacteriophage workers / Andrew M. Kropinski.
9781588296825 (v.1) (hbk.) 1588296822 (v.1) (hbk.) 9781603275644 (v. 2) (hbk.) 1603275649 (v. 2) (hbk.) 9781603271646 (v. 1) (eISBN) 1603271643 (v. 1) (eISBN) 9781603275651 (v. 2) (eISBN) 1603275657 (v. 2) (eISBN)
2008939449
GBA894232 bnb
101493772 DNLM 20090091124
Bacteriophages--Research--Methodology.
Bacteriophages.
Bacteriophages--physiology--Laboratory Manuals.
Bacteriophages--isolation & purification--Laboratory Manuals.
Genomics--methods--Laboratory Manuals.
Bactériophages.
QR342 / .B34 2009
576.6482 CLO
QR342 / .B339 2009
W1 ME9616J v. 501-502 2009 QW 25.5.B2 / B131 2009